All agent skills (63,961) - page 537 of 640

Backend DevBuild production-grade backend services with proper error handling and API design.3 installs

Background ProcessHousekeeping guidance for launching background processes cleanly: avoiding stale processes and port conflicts, verifying startup, and choosing signals.3 installs

Backup ProjectHelps with ai & agent building tasks.3 installs

Bayesian WorkflowBuilding Bayesian models in PyMC and ArviZ with guardrails for priors, sampling, and diagnostics.3 installs

Beautiful SlidesBuild or edit an editable PowerPoint deck from a brief with disciplined layout and a must-include audit.3 installs

BeautifyHelps with ai & agent building tasks.3 installs

Before And AfterHelps with ai & agent building tasks.3 installs

Better AuthHelps with security tasks during AI-assisted development.3 installs

Bibliography Management GuideHelps with ai & agent building tasks during AI-assisted development.3 installs

Billing Money RulesEnforces Helpline's money-handling rules for code under services/billing: integer cents only, centralized plan economics, and BillingError on violations.3 installs

Bio Alignment FilteringFilter sequencing alignments by SAM flags, mapping quality, and genomic regions using samtools view and pysam to extract or clean reads.3 installs

Bio Alignment PairwisePerform pairwise sequence alignment with Biopython's PairwiseAligner using global (Needleman-Wunsch) or local (Smith-Waterman) algorithms to score similarity.3 installs

Bio Batch ProcessingProcess many sequence files in batch with Biopython SeqIO, iterating directories to merge, split, or automate operations across FASTA and FASTQ files.3 installs

Bio Bedgraph HandlingCreate, manipulate, and convert bedGraph coverage/signal files (to and from bigWig) for genome-browser visualization of ChIP-seq, ATAC-seq, or RNA-seq.3 installs

Bio Chip Seq Super EnhancersIdentify super-enhancers from H3K27ac ChIP-seq data using the ROSE (Rank Ordering of Super-Enhancers) tool for cell-identity and cancer regulatory analysis.3 installs

Bio Clinical Databases Dbsnp QueriesQuery dbSNP for rsID lookups, variant annotations, and cross-references using myvariant.info and NCBI Entrez to map rsIDs to genomic coordinates.3 installs

Bio Clinical Databases Variant PrioritizationFilter and prioritize sequencing variants by pathogenicity, population frequency, and clinical evidence for rare-disease exome/genome analysis using pandas.3 installs

Bio Clip Seq Clip Peak CallingCall protein-RNA binding-site peaks from CLIP-seq data using CLIPper, PureCLIP, or Piranha to identify RNA-binding-protein sites from aligned reads.3 installs

Bio Copy Number Gatk CnvCall copy-number variants with the GATK best-practices workflow, supporting somatic tumor-normal and germline CNV detection from WGS or WES data.3 installs

Bio De Edger BasicsRun differential expression on RNA-seq counts with edgeR's quasi-likelihood F-test, from DGEList creation through normalization, dispersion, and testing.3 installs

Bio De ResultsFilter, annotate, and export differential expression results from DESeq2 or edgeR, applying significance thresholds and adding gene annotations.3 installs

Bio Entrez LinkFind cross-references between NCBI databases with Biopython Bio.Entrez ELink, navigating from genes to proteins, sequences to publications, and related records.3 installs

Bio Epitranscriptomics M6anet AnalysisDetect m6A modifications from Oxford Nanopore direct RNA sequencing using m6Anet, without immunoprecipitation.3 installs

Bio Format ConversionConvert between sequence file formats such as FASTA, FASTQ, GenBank, and EMBL using Biopython Bio.SeqIO.3 installs

Bio Gatk Variant CallingCall germline SNPs and indels with GATK HaplotypeCaller following Best Practices, including GVCF cohorts, joint genotyping, and VQSR.3 installs

Bio Genome Assembly ScaffoldingScaffold contigs into chromosome-level assemblies with Hi-C data using YaHS, 3D-DNA, and SALSA2, validated with BUSCO and contact maps.3 installs

Bio Genome Intervals Bigwig TracksCreate and read bigWig browser tracks, convert bedGraph to bigWig, and extract signal at regions using UCSC tools and pyBigWig.3 installs

Bio Geo DataQuery NCBI Gene Expression Omnibus (GEO) for expression datasets and link GEO series to SRA runs using Biopython Bio.Entrez.3 installs

Bio Imaging Mass Cytometry Cell SegmentationSegment cells from multiplexed tissue images using deep learning (Cellpose, Mesmer) and classical methods to extract single-cell data from IMC or MIBI.3 installs

Bio Imaging Mass Cytometry PhenotypingAssign cell types from marker expression in IMC data using manual gating, clustering, and automated classification with scanpy.3 installs

Bio Longread QcQuality-control long-read sequencing data with NanoPlot, NanoStat, and chopper: generate reports and filter reads by length and quality.3 installs

Bio Long Read Sequencing Clair3 VariantsCall germline SNPs and small indels from ONT or PacBio alignments using the deep-learning caller Clair3.3 installs

Bio Metabolomics Statistical AnalysisRun statistical analysis on metabolomics data with univariate testing, multivariate methods (PCA, PLS-DA), and biomarker discovery using mixOmics.3 installs

Bio Metabolomics Xcms PreprocessingPreprocess LC-MS/MS metabolomics data with the XCMS3 workflow: peak detection, retention time alignment, correspondence, and gap filling.3 installs

Bio Methylation CallingExtract methylation calls from Bismark BAM files with bismark_methylation_extractor, generating per-cytosine CpG, CHG, and CHH reports.3 installs

Bio Methylation Dmr DetectionDetect differentially methylated regions with methylKit tiles, bsseq BSmooth, and DMRcate across experimental conditions or cell types.3 installs

Bio Methylation MethylkitAnalyze single-base DNA methylation with methylKit in R: import Bismark coverage files, filter, normalize, and compare samples statistically.3 installs

Bio Paired End FastqHandle paired-end FASTQ R1/R2 files with Biopython: synchronize pairs, interleave or deinterleave, and filter paired reads.3 installs

Bio Pdb Geometric AnalysisMeasure distances, angles, dihedrals, RMSD, and SASA on protein structures with Biopython Bio.PDB in Python.3 installs

Bio Pdb Structure IoRead, download from RCSB, and write protein structure files in PDB, mmCIF, and MMTF formats with Biopython Bio.PDB.3 installs

Bio Population Genetics Association TestingRun GWAS case-control and quantitative-trait association tests with covariates using PLINK 2.0 --glm.3 installs

Bio Population Genetics Linkage DisequilibriumCalculate LD statistics (r-squared, D-prime) and prune correlated variants using PLINK and scikit-allel.3 installs

Bio Population Genetics Plink BasicsConvert genotype file formats and apply MAF, call-rate, and HWE quality filters using PLINK 1.9 and 2.0.3 installs

Bio Population Genetics Population StructureAnalyze population structure with PCA and ADMIXTURE ancestry analysis using PLINK and ADMIXTURE.3 installs

Bio Population Genetics Scikit Allel AnalysisAnalyze population genetics in Python with scikit-allel: read VCF, compute allele frequencies, diversity, and PCA.3 installs

Bio Population Genetics Selection StatisticsCompute selection statistics like Fst, Tajima's D, and iHS to detect selective sweeps using scikit-allel and vcftools.3 installs

Bio Read Alignment Bowtie2 AlignmentAlign short reads (ChIP-seq, ATAC-seq) to a reference genome using Bowtie2 in local or end-to-end mode.3 installs

Bio Read Alignment Bwa AlignmentAlign DNA short reads to a reference genome using bwa-mem2 with read-group tags for downstream GATK.3 installs

Bio Read Alignment Hisat2 AlignmentAlign RNA-seq reads with the memory-efficient splice-aware aligner HISAT2 when STAR needs too much memory.3 installs

Bio Read Alignment Star AlignmentAlign RNA-seq reads with STAR splice-aware alignment, including two-pass mode for novel splice junctions.3 installs

Bio Read Qc Adapter TrimmingRemove sequencing adapters from FASTQ reads using Cutadapt or Trimmomatic before short-read alignment.3 installs

Bio Read Qc Contamination ScreeningScreen FASTQ reads against multiple genomes with FastQ Screen to detect contamination or sample swaps.3 installs

Bio Read Qc Quality FilteringFilter reads by quality, length, and N content with sliding-window trimming using Trimmomatic and fastp.3 installs

Bio Read Qc Quality ReportsGenerate and aggregate FASTQ quality reports using FastQC and MultiQC to assess raw sequencing data.3 installs

Bio Read Qc Umi ProcessingExtract and deduplicate reads by Unique Molecular Identifier using umi_tools for accurate molecule counting.3 installs

Bio Reporting Quarto ReportsBuild reproducible scientific reports, slides, and sites with Quarto across R, Python, Julia, and Observable JS.3 installs

Bio Restriction Enzyme SelectionSelect restriction enzymes by cut frequency, overhang, or compatible ends for cloning using Biopython Bio.Restriction.3 installs

Bio Restriction Fragment AnalysisPredict restriction digest fragment sizes and simulate gel patterns using Biopython Bio.Restriction in Python.3 installs

Bio Restriction MappingCreate restriction maps of enzyme cut positions on a DNA sequence using Biopython Bio.Restriction in Python.3 installs

Bio Restriction SitesFind restriction enzyme cut sites in a DNA sequence with single enzymes or batches using Biopython Bio.Restriction.3 installs

Bio Structural Biology Alphafold PredictionsDownload AlphaFold predicted protein structures from the EBI database and analyze pLDDT confidence and PAE matrices.3 installs

Bio Structural Biology Modern Structure PredictionPredict protein structures using AlphaFold3, ESMFold, Chai-1, Boltz-1, or ColabFold and compare the results.3 installs

Bio Variant Calling Joint CallingPerform joint genotype calling across multiple samples with GATK CombineGVCFs, GenomicsDBImport, and GenotypeGVCFs.3 installs

Bio Vcf StatisticsGenerate variant statistics, sample concordance, and quality metrics from VCF files using bcftools stats and gtcheck.3 installs

Bio Workflow Management Nextflow PipelinesCreate scalable, containerized bioinformatics pipelines with Nextflow DSL2 supporting Docker, Singularity, and cloud execution.3 installs

Bio Workflow Management Wdl WorkflowsCreate portable bioinformatics pipelines with Workflow Description Language using Cromwell or miniwdl engines.3 installs

Bio Workflows Chipseq PipelineRun an end-to-end ChIP-seq workflow from FASTQ through QC, Bowtie2 alignment, MACS3 peak calling, and peak annotation.3 installs

Bio Workflows Clip PipelineRun an end-to-end CLIP-seq analysis from FASTQ through UMI handling, alignment, peak calling, and motif enrichment.3 installs

Bio Workflows Gwas PipelineRun an end-to-end GWAS from VCF through PLINK QC, population structure correction, and association testing.3 installs

Bio Workflows Imc PipelineRun an end-to-end imaging mass cytometry workflow from raw acquisitions to spatial single-cell analysis with steinbock.3 installs

Bio Workflows Metabolomics PipelineRun an end-to-end LC-MS metabolomics workflow from raw MS data through XCMS preprocessing to pathway analysis.3 installs

Bio Workflows Methylation PipelineRun an end-to-end bisulfite sequencing workflow from FASTQ to differentially methylated regions with Bismark and methylKit.3 installs

Bio Workflows Multiome PipelineRun an end-to-end multiome workflow for joint scRNA-seq and scATAC-seq analysis with WNN integration in Seurat/Signac.3 installs

Bio Workflows Multi Omics PipelineRun an end-to-end multi-omics integration workflow across transcriptomics, proteomics, and metabolomics with MOFA2 and mixOmics.3 installs

Bio Workflows Spatial PipelineRun an end-to-end spatial transcriptomics workflow for Visium/Xenium data covering preprocessing, domain detection, and visualization with Squidpy.3 installs

Bio Workflows Tcr PipelineRun an end-to-end TCR/BCR repertoire analysis from FASTQ to clonotype diversity metrics using MiXCR and VDJtools.3 installs

Blender Syntax OperatorsHelps with ai & agent building tasks.3 installs

Blog PostTurn a topic or brief into a long-form post plus social and multimedia adaptations via an 8-phase pipeline.3 installs

Bmad Os Changelog SocialTurn the latest changelog entry into ready-to-post release announcements for Discord, Twitter/X, Facebook, and LinkedIn.3 installs

Bookkeeping Agency SkillHelps with ai & agent building tasks.3 installs

Brand Guidelines Generatorcreate and maintain brand style guides for consistent visual identity3 installs

Brand Identity GeneratorHelps with ai & agent building tasks.3 installs

Briefing NoteProduce a decision-ready 1-2 page policy briefing note in a standard government or consulting format from a topic and audience.3 installs

Browser Automationautomate web browser interactions, scraping, testing, and workflow automation3 installs

BrregSearch and retrieve Norwegian company data (org numbers, status, industry, location) from the official Brreg open API.3 installs

Brutal Honesty ReviewHelps with ai & agent building tasks.3 installs

BuffettHelps with ai & agent building tasks during AI-assisted development.3 installs

Bug Reporting ExcellenceHelps with ai & agent building tasks.3 installs

Bugs FlowInvestigate, fix, and verify a specific bug from symptoms, logs, or Sentry issues using a single bug doc.3 installs

Building Webapp React ComponentsGuides creating and modifying React pages, components, headers, and footers in a Salesforce web app using shadcn UI and Tailwind CSS.3 installs

But AgentlogRecover prior agent work and branch context by skimming captured `but agentlog` sessions for a GitButler branch, review, PR, or change.3 installs

Byted Emr SkillsManages Volcengine EMR big-data clusters, Serverless queues, compute groups, Spark/Presto/Ray jobs, logs, monitoring, and agent-based job diagnosis.3 installs

Byted Link ReaderHelps with ai & agent building tasks.3 installs

Byted Marketing Agent Inspiration InsightHelps with marketing & seo tasks.3 installs

Byted Marketing Agent Trending ListHelps with marketing & seo tasks.3 installs

Byted Podcast GenHelps with ai & agent building tasks.3 installs

Byted Seedance Video GenerateHelps with ai & agent building tasks.3 installs

Byted Sms SenderHelps with ai & agent building tasks.3 installs

Canvas DesignHelps with ai & agent building tasks during AI-assisted development.3 installs

Cartridge PaymasterSets up Cartridge paymasters via the slot CLI to sponsor Starknet transaction fees, managing budgets, sponsorship policies, and transaction monitoring for gasless UX.3 installs

All agent skills - page 537 of 640